Expression, characterization and antivascular activity of amino acid sequence repeating collagen hexadecapeptide

Wenjing Yan; Changjin Huang; Yumiao Yan; Pan Wang; Weigang Yuwen; Chenhui Zhu; Rongzhan Fu; Zhiguang Duan; Daidi Fan

doi:10.1016/j.ijbiomac.2024.131886

Expression, characterization and antivascular activity of amino acid sequence repeating collagen hexadecapeptide

Int J Biol Macromol. 2024 Apr 25:131886. doi: 10.1016/j.ijbiomac.2024.131886. Online ahead of print.

Authors

Wenjing Yan¹, Changjin Huang², Yumiao Yan³, Pan Wang¹, Weigang Yuwen⁴, Chenhui Zhu¹, Rongzhan Fu¹, Zhiguang Duan⁵, Daidi Fan⁶

Affiliations

¹ Engineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710069, China.
² Department of Craniomaxillofacial Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
³ Xi'an Gaoxin No.1 High School, Xi'an, China.
⁴ Shaanxi Giant Biotechnology Co., Ltd., Xi'an 710065, Shaanxi, China.
⁵ Engineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710069, China. Electronic address: duanzhiguang@nwu.edu.cn.
⁶ Engineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710069, China. Electronic address: fandaidi@nwu.edu.cn.

PMID: 38677696
DOI: 10.1016/j.ijbiomac.2024.131886

Abstract

Type V collagen is an essential component of the extracellular matrix (ECM), and its remodeling releases specific protein fragments that can specifically inhibit endothelial cell responses such as proliferation, migration, and invasion. In this study, we have successfully constructed two engineered strains of Pichia pastoris capable of producing recombinant collagen through a new genetic engineering approach. Through high-density fermentation, the expression of 1605 protein and 1610 protein could reach 2.72 g/L and 4.36 g/L. With the increase of repetition times, the yield also increased. Bioactivity analysis showed that recombinant collagen could block the angiogenic effect of FGF-2 on endothelial cells by eliminating FGF-2-induced endothelial cell migration and invasion. Collectively, the recombinant proteins we successfully expressed have a wide range of potential for inhibiting angiogenesis in the biomaterials and biomedical fields.

Keywords: FGF-2; Inhibit angiogenesis; Pichia pastoris; Recombinant collagen; Sequence repetition.