Construction of Fu brick tea polysaccharide-cold plasma modified alginate microgels for probiotic delivery: Enhancing viability and colonization

Rui Sun; Ying Wang; Zhongyi Lv; Hairui Li; Shirui Zhang; Qilei Dang; Xubo Zhao; Tianli Yue; Yahong Yuan

doi:10.1016/j.ijbiomac.2024.131899

Construction of Fu brick tea polysaccharide-cold plasma modified alginate microgels for probiotic delivery: Enhancing viability and colonization

Int J Biol Macromol. 2024 Apr 25;268(Pt 1):131899. doi: 10.1016/j.ijbiomac.2024.131899. Online ahead of print.

Authors

Rui Sun¹, Ying Wang¹, Zhongyi Lv¹, Hairui Li², Shirui Zhang², Qilei Dang³, Xubo Zhao⁴, Tianli Yue⁵, Yahong Yuan⁶

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, YangLing 712100, Shaanxi, China.
² College of Food Science and Technology, Northwest University, Xi'an 710069, China.
³ Qin Chuangyuan Fu Tea Culture Innovation Center, Xi'an 713700, China.
⁴ College of Food Science and Engineering, Northwest A&F University, YangLing 712100, Shaanxi, China. Electronic address: zhxubo@nwafu.edu.cn.
⁵ College of Food Science and Engineering, Northwest A&F University, YangLing 712100, Shaanxi, China; College of Food Science and Technology, Northwest University, Xi'an 710069, China. Electronic address: yuetl@nwafu.edu.cn.
⁶ College of Food Science and Engineering, Northwest A&F University, YangLing 712100, Shaanxi, China; College of Food Science and Technology, Northwest University, Xi'an 710069, China. Electronic address: yyh324@126.com.

PMID: 38677703
DOI: 10.1016/j.ijbiomac.2024.131899

Abstract

Emerging food processing technologies provide broader avenues for enhancing probiotic delivery systems. In this study, the new Fu brick tea polysaccharide (FBTP) was extracted and combined with cold plasma-modified alginate nano-montmorillonite (AMT) to prepare microgels by ionic gelation to improve the viability of encapsulated Lactobacillus kefiranofaciens JKSP109. Results showed that cold plasma treatment for 3 min changed the surface charge of AMT biopolymer solution, and FBTP addition reduced the particle size to the lowest of 223 ± 5.50 nm. Morphological analysis showed that the AMT treated with cold plasma for 3 min and FBTP (C3AMT + FBTP) formed a dense microgel through electrostatic interaction, and the probiotics were randomly distributed in their internal polysaccharide network, as well as the interlayer and surrounding of nanoparticles. The probiotics immobilized in C3AMT + FBTP microgel exhibited the highest viability (8.48 ± 0.03 log CFU/g) and colonic colonization after exposure to simulated gastrointestinal conditions. In addition, the good antioxidant activity of FBTP reduced the loss of probiotic viability during storage, with only 2.58 log CFU/g decreased after 4 weeks. Therefore, such probiotic products enriched with natural bioactive ingredients can be developed as a potential functional food additive.

Keywords: Alginate; Cold plasma; Fu brick tea polysaccharide; Lactobacillus kefiranofaciens; Probiotics delivery.