Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

Seungbin Park; Megan Lipton; Yujiao J Sun; Maria Dadarlat

doi:10.1016/j.xpro.2024.103027

Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

STAR Protoc. 2024 Apr 27;5(2):103027. doi: 10.1016/j.xpro.2024.103027. Online ahead of print.

Authors

Seungbin Park¹, Megan Lipton¹, Yujiao J Sun², Maria Dadarlat³

Affiliations

¹ Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
² Institute of Ophthalmology, University College London, 11-43 Bath Street, EC1V 9EL London, UK.
³ Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA. Electronic address: mdadarla@purdue.edu.

Abstract

Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head-fixed recordings. For complete details on the use and execution of this protocol, please refer to Dadarlat et al.¹.

Keywords: Biotechnology and bioengineering; Neuroscience.