Improving resilience to high temperature in drought: water replenishment enhances sucrose and amino acid metabolisms in maize grain

Xinglong Wang; Junhao Wang; Yupeng Zhu; Ziren Qu; Xiwei Liu; Pu Wang; Qingfeng Meng

doi:10.1111/tpj.16783

Improving resilience to high temperature in drought: water replenishment enhances sucrose and amino acid metabolisms in maize grain

Plant J. 2024 Apr 28. doi: 10.1111/tpj.16783. Online ahead of print.

Authors

Xinglong Wang^{1

2}, Junhao Wang¹, Yupeng Zhu¹, Ziren Qu¹, Xiwei Liu³, Pu Wang¹, Qingfeng Meng¹

Affiliations

¹ College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
² College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, China.
³ Key Laboratory of Crop Physiology and Ecology, Center for Crop Management and Farming System, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Beijing, 100081, China.

PMID: 38678590
DOI: 10.1111/tpj.16783

Abstract

Heat stress poses a significant threat to maize, especially when combined with drought. Recent research highlights the potential of water replenishment to ameliorate grain weight loss. However, the mitigating mechanisms of heat in drought stress, especially during the crucial early grain-filling stage, remain poorly understood. We investigated the mechanism for mitigating heat in drought stress by water replenishment from the 12th to the 32nd days after silking in a controlled greenhouse experiment (Exp. I) and field trial (Exp. II). A significant reduction in grain weight was observed in heat stress compared to normal conditions. When water replenishment was applied to increase soil water content (SWC) under heat stress, the grain yield exhibited a notable increase ranging from 28.4 to 76.9%. XY335 variety was used for transcriptome sequencing to analyze starch biosynthesis and amino acid metabolisms in Exp. I. With water replenishment, the transcripts of genes responsible for trehalose 6-phosphate phosphates (TPP), alpha-trehalase (TRE), ADP-glcpyrophosphorylase, and starch synthase activity were stimulated. Additionally, the expression of genes encoding TPP and TRE contributed to an enhanced conversion of trehalose to glucose. This led to the conversion of sucrose from glucose-1-phosphate to ADP-glucose and ADP-glucose to amylopectin, ultimately increasing starch production by 45.1%. Water replenishment to boost SWC during heat stress also elevated the levels of essential amino acids in maize, including arginine, serine, tyrosine, leucine, glutamic acid, and methionine, providing valuable support to maize plants in adversity. Field trials further validated the positive impact of water replenishment on SWC, resulting in a notable increase in grain yield ranging from 7.1 to 9.2%. This study highlights the vital importance of adapting to abiotic stress and underscores the necessity of developing strategies to counteract its adverse effects on crop yield.

Keywords: grain weight; heat stress; maize; starch biosynthesis; transcriptome; water replenishment.

Abstract

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