MHC class I-dressing is mediated via phosphatidylserine recognition and is enhanced by polyI:C

Arisa Hori; Saori Toyoura; Miyu Fujiwara; Ren Taniguchi; Yasutaka Kano; Tomoyoshi Yamano; Rikinari Hanayama; Masafumi Nakayama

doi:10.1016/j.isci.2024.109704

MHC class I-dressing is mediated via phosphatidylserine recognition and is enhanced by polyI:C

iScience. 2024 Apr 10;27(5):109704. doi: 10.1016/j.isci.2024.109704. eCollection 2024 May 17.

Authors

Arisa Hori¹, Saori Toyoura¹, Miyu Fujiwara¹, Ren Taniguchi¹, Yasutaka Kano¹, Tomoyoshi Yamano^{2

3}, Rikinari Hanayama^{2

3}, Masafumi Nakayama^{1

4}

Affiliations

¹ Laboratory of Immunology and Microbiology, College of Pharmaceutical Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan.
² Department of Immunology, Kanazawa University Graduate School of Medical Sciences, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8640, Japan.
³ WPI Nano Life Science Institute (NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan.
⁴ Research Center for Animal Life Science, Shiga University of Medical Sciences, Seta, Tsukinowa-cho, Otsu, Shiga 520-2192, Japan.

Abstract

In addition to cross-presentation, cross-dressing plays an important role in the induction of CD8⁺ T cell immunity. In the process of cross-dressing, conventional dendritic cells (DCs) acquire major histocompatibility complex class I (MHCI) from other cells and subsequently prime CD8⁺ T cells via the pre-formed antigen-MHCI complexes without antigen processing. However, the mechanisms underlying the cross-dressing pathway, as well as the relative contributions of cross-presentation and cross-dressing to CD8⁺ T cell priming are not fully understood. Here, we demonstrate that DCs rapidly acquire MHCI-containing membrane fragments from dead cells via the phosphatidylserine recognition-dependent mechanism for cross-dressing. The MHCI dressing is enhanced by a TLR3 ligand polyinosinic-polycytidylic acid (polyI:C). Further, polyI:C promotes not only cross-presentation but also cross-dressing in vivo. Taken together, these results suggest that cross-dressing as well as cross-presentation is involved in inflammatory diseases associated with cell death and type I IFN production.

Keywords: Cell biology; Components of the immune system; Immunology.