Soluble expression of recombinant coagulation factor IX protein using Escherichia coli

Byoung-Hee Park; Hanool Yun; Hee-Jin Jeong

doi:10.1016/j.bbrep.2024.101714

Soluble expression of recombinant coagulation factor IX protein using Escherichia coli

Biochem Biophys Rep. 2024 Apr 18:38:101714. doi: 10.1016/j.bbrep.2024.101714. eCollection 2024 Jul.

Authors

Byoung-Hee Park¹, Hanool Yun², Hee-Jin Jeong²

Affiliations

¹ Industry-Academia Cooperation Foundation, Hongik University, 2639 Sejong-ro, Sejong-si, 30016, South Korea.
² Department of Biological and Chemical Engineering, Hongik University, 2639 Sejong-ro, Sejong-si, 30016, South Korea.

Abstract

Hemophilia B is a congenital bleeding disorder caused by factor IX (FIX) deficiency. Generation of recombinant FIX (rFIX) is required for detecting a Hemophilia B indicator, anti-FIX antibody. In this study, we described a method for producing recombinant FIX (rFIX) using Escherichia coli. We constructed a FIX-expressing plasmid without a fusion tag protein-encoding gene and produced rFIX as a soluble form within five days. Dose-dependent curve was obtained from ELISA using anti-FIX antibody, indicating that the rFIX can be used as an antigen to detect anti-FIX antibody with high affinity and sensitivity.

Keywords: Escherichia coli; Factor IX; Hemophilia; Protein expression; Recombinant protein.