Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications

Yinze Shi; Xueyang Yang; Jie Min; Wen Kong; Xiang Hu; Jiaoyue Zhang; Lulu Chen

doi:10.3389/fendo.2024.1343255

Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications

Front Endocrinol (Lausanne). 2024 Apr 12:15:1343255. doi: 10.3389/fendo.2024.1343255. eCollection 2024.

Authors

Yinze Shi^#^{1

2}, Xueyang Yang^#^{1

2}, Jie Min^{1

2}, Wen Kong^{1

2}, Xiang Hu^{1

2}, Jiaoyue Zhang^{1

2}, Lulu Chen^{1

2}

Affiliations

¹ Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
² Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorders, Wuhan, China.

^# Contributed equally.

Abstract

Stem cell-based therapies exhibit considerable promise in the treatment of diabetes and its complications. Extensive research has been dedicated to elucidate the characteristics and potential applications of adipose-derived stromal/stem cells (ASCs). Three-dimensional (3D) culture, characterized by rapid advancements, holds promise for efficacious treatment of diabetes and its complications. Notably, 3D cultured ASCs manifest enhanced cellular properties and functions compared to traditional monolayer-culture. In this review, the factors influencing the biological functions of ASCs during culture are summarized. Additionally, the effects of 3D cultured techniques on cellular properties compared to two-dimensional culture is described. Furthermore, the therapeutic potential of 3D cultured ASCs in diabetes and its complications are discussed to provide insights for future research.

Keywords: adipose-derived stem/stromal cells; diabetes mellitus; diabetic foot ulcer; monolayer culture; three-dimensional culture.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue* / cytology
Animals
Cell Culture Techniques / methods
Cell Culture Techniques, Three Dimensional / methods
Cell Differentiation
Diabetes Complications / therapy
Diabetes Mellitus* / therapy
Humans
Mesenchymal Stem Cells / cytology

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by the National Natural Science Foundation of China (82170822, 82070809, 82300895, and 81900734).