Internal standard optimization advances sensitivity and robustness of ratiometric detection method

Yuning Jiang; Jiaying Cao; Sen Hu; Tao Cheng; Hanyu Wang; Xiaoyu Guo; Ye Ying; Xinling Liu; Feng Wang; Ying Wen; Yiping Wu; Haifeng Yang

doi:10.1039/d4an00266k

Internal standard optimization advances sensitivity and robustness of ratiometric detection method

Analyst. 2024 May 13;149(10):2806-2811. doi: 10.1039/d4an00266k.

Authors

Yuning Jiang¹, Jiaying Cao¹, Sen Hu¹, Tao Cheng¹, Hanyu Wang¹, Xiaoyu Guo¹, Ye Ying¹, Xinling Liu¹, Feng Wang¹, Ying Wen¹, Yiping Wu¹, Haifeng Yang¹

Affiliation

¹ The Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, and Shanghai Key Laboratory of Rare Earth Functional Materials, College of Environmental and Geographic Sciences, Shanghai Normal University, Shanghai 200234, China. yipingwu@shnu.edu.cn.

PMID: 38683246
DOI: 10.1039/d4an00266k

Abstract

We design a p-aminothiophenol (pATP) modified Au/ITO chip to determine nitrite ions in lake water by a ratiometric surface-enhanced Raman scattering (SERS) method based on nitrite ions triggering the transformation of pATP to p,p'-dimercaptoazobenzene (DMAB). Intriguingly, by using the SERS peak (at 1008 cm^-1) from benzoic ring deforming as an internal standard instead of the traditional peak at 1080 cm^-1, the detection sensitivity of the method was improved 10 times.