Characterization of FBA genes in potato (Solanum tuberosum L.) and expression patterns in response to light spectrum and abiotic stress

Ting Li; Xinyue Hou; Zhanglun Sun; Bin Ma; Xingxing Wu; Tingting Feng; Hao Ai; Xianzhong Huang; Ruining Li

doi:10.3389/fgene.2024.1364944

Characterization of FBA genes in potato (Solanum tuberosum L.) and expression patterns in response to light spectrum and abiotic stress

Front Genet. 2024 Apr 12:15:1364944. doi: 10.3389/fgene.2024.1364944. eCollection 2024.

Authors

Ting Li^#¹, Xinyue Hou^#¹, Zhanglun Sun¹, Bin Ma², Xingxing Wu¹, Tingting Feng¹, Hao Ai¹, Xianzhong Huang¹, Ruining Li¹

Affiliations

¹ Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, China.
² Country College of Life Sciences, Shihezi University, Shihezi, China.

^# Contributed equally.

Abstract

Fructose-1, 6-bisphosphate aldolase (FBA) plays vital roles in plant growth, development, and response to abiotic stress. However, genome-wide identification and structural characterization of the potato (Solanum tuberosum L.) FBA gene family has not been systematically analyzed. In this study, we identified nine StFBA gene members in potato, with six StFBA genes localized in the chloroplast and three in the cytoplasm. The analysis of gene structures, protein structures, and phylogenetic relationships indicated that StFBA genes were divided into Class I and II, which exhibited significant differences in structure and function. Synteny analysis revealed that segmental duplication events promoted the expansion of the StFBA gene family. Promoter analysis showed that most StFBA genes contained cis-regulatory elements associated with light and stress responses. Expression analysis showed that StFBA3, StFBA8, and StFBA9 showing significantly higher expression levels in leaf, stolon, and tuber under blue light, indicating that these genes may improve photosynthesis and play an important function in regulating the induction and expansion of microtubers. Expression levels of the StFBA genes were influenced by drought and salt stress, indicating that they played important roles in abiotic stress. This work offers a theoretical foundation for in-depth understanding of the evolution and function of StFBA genes, as well as providing the basis for the genetic improvement of potatoes.

Keywords: Solanum tuberosum; abiotic stress; fructose-1, 6-bisphosphate aldolase; gene expression; genome-wide identification; light spectrum.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This research was funded by the Natural Science Foundation of Anhui (No. 2108085QC125), the Key Project of Anhui Provincial Education Department (Nos 2022AH051627 and 2023AH051886), the Talent Introduction Start-up Fund Project of Anhui Science and Technology University (No. NXYJ202102), the National Innovation and Entrepreneurship Training Program for College Students (No. 202310879007), and the Provincial Graduate Full English/Bilingual Demonstration Course (No. 2022qyw/sysfkc040). The funding institution was not involved in the design of the study, collection, analysis, and interpretation of data, and in writing the manuscript.