The cGAS/STING signaling pathway is involved in sevoflurane induced neuronal necroptosis via regulating microglia M1 polarization

Cell Signal. 2024 Jul:119:111195. doi: 10.1016/j.cellsig.2024.111195. Epub 2024 Apr 28.

Abstract

Objective: The specific mechanisms of sevoflurane-induced neurotoxicity are still undetermined. The aim of the current study was to investigate the role of the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway in sevoflurane-induced neuronal necroptosis.

Methods: BV2 microglial cells were divided into a control group and a 4% sevoflurane exposure group. Western blotting was used to detect expression of the M1 polarization marker inducible nitric oxide synthase (iNOS). RNA was collected for RNA sequencing analysis. After STING knockdown in microglia, western blotting was performed to examine expression of the pro-inflammatory markers CD16 and CD32. The tumor necrosis factor-α (TNF-α) level in media was detected using an enzyme-linked immunosorbent assay. BV2 microglia conditioned media was collected to incubate HT22 neuronal cells, and their cell activity was measured using a CCK8 assay. Calcium was observed by fluorescence. Western blotting was performed to evaluate receptor-interacting protein kinase 1 (RIPK1), RIPK3, and mixed lineage kinase domain-like (MLKL) expression. Neuronal necroptosis rate were detected using flow cytometry.

Results: Sevoflurane exposure promoted microglial M1 polarization. The cGAS/STING pathway was screened and identified by RNA sequencing analysis of sevoflurane-exposed microglia and the control group. Compared with the control group, STING knockdown in microglia rescued the amoeboid morphology, inhibited TNF-α release, and significantly decreased iNOS, CD16, and CD32 expression. Moreover, calcium ions and necroptosis within neurons were decreased, and RIPK1, RIPK3, and p-MLKL expression was markedly decreased in microglia media culture with STING knockdown.

Conclusion: These results suggest that sevoflurane can regulate microglial M1 polarization by activating the cGAS/STING signaling pathway and increasing immune factor release, thus accelerating the neuronal necroptosis induced by calcium overload.

Keywords: Calcium; Microglia; Neuronal necroptosis; Sevoflurane; cGAS/STING signaling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Membrane Proteins* / genetics
  • Membrane Proteins* / metabolism
  • Mice
  • Microglia* / drug effects
  • Microglia* / metabolism
  • Necroptosis* / drug effects
  • Neurons* / drug effects
  • Neurons* / metabolism
  • Nucleotidyltransferases* / metabolism
  • Sevoflurane* / pharmacology
  • Signal Transduction* / drug effects
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Sevoflurane
  • Membrane Proteins
  • Sting1 protein, mouse
  • cGAS protein, mouse
  • Nucleotidyltransferases
  • Tumor Necrosis Factor-alpha