IGF2BP2 regulates the proliferation and migration of endometrial stromal cells through the PI3K/AKT/mTOR signaling pathway in Hu sheep

Kaiping Deng; Xiaodan Li; Zhipeng Liu; Yalong Su; Xuan Sun; Wurilege Wei; Yixuan Fan; Yanli Zhang; Feng Wang

doi:10.1093/jas/skae129

IGF2BP2 regulates the proliferation and migration of endometrial stromal cells through the PI3K/AKT/mTOR signaling pathway in Hu sheep

J Anim Sci. 2024 May 10:skae129. doi: 10.1093/jas/skae129. Online ahead of print.

Authors

Kaiping Deng^{1

2}, Xiaodan Li^{1

2}, Zhipeng Liu³, Yalong Su^{1

2}, Xuan Sun^{1

2}, Wurilege Wei^{1

4}, Yixuan Fan^{1

2}, Yanli Zhang^{1

2}, Feng Wang^{1

2}

Affiliations

¹ Hu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China.
² Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing, 210095, China.
³ Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, Nanjing, 210095, China.
⁴ Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences.

PMID: 38727196
DOI: 10.1093/jas/skae129

Abstract

Insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2), a significant member of the conserved RNA-binding protein family, plays various roles in numerous physiological and pathological processes. However, the specific function of IGF2BP2 in regulating endometrial function in sheep remains largely unknown. In this study, we observed a significant upregulation in IGF2BP2 mRNA abundance in the endometrium during the luteal phase (LP) compared to the follicular phase (FP) in Hu sheep. The knockdown of IGF2BP2 resulted in accelerated cell proliferation and migration of Hu sheep endometrial stromal cells (ESCs). Moreover, RNA sequencing analysis revealed that genes with significantly altered expression in IGF2BP2 knockdown cells were predominantly enriched in endometrial receptivity-related signaling pathways, such as cytokine-cytokine receptor interaction, NOD-like receptor, PI3K-AKT, and JAK-STAT signaling pathway. Additionally, the knockdown of IGF2BP2 significantly increased the expression of matrix metalloprotein 9 (MMP9), vascular endothelial growth factor (VEGF), and prolactin (PRL) in ESCs. The knockdown of IGF2BP2 was also observed to stimulate the PI3K/AKT/mTOR pathway by upregulating integrin β4 (ITGB4) expression. Notably, the downregulation of ITGB4 attenuates IGF2BP2 knockdown-induced facilitation of proliferation and migration of Hu sheep ESCs by inhibiting the PI3K/AKT/mTOR pathway. Collectively, these findings highlight the important role of IGF2BP2 in regulating endometrial function, particularly through the modulation of ESC proliferation and migration via the PI3K/AKT/mTOR pathway.

Keywords: Hu sheep; IGF2BP2; PI3K/AKT/mTOR pathway; endometrial function; endometrial stromal cells.