Clinical presentation, antimicrobial resistance, and treatment outcomes of Aeromonas human infections: A 14-year retrospective study and comparative genomics of two isolates from fatal cases

Roberto Pineda-Reyes; Blake H Neil; Joseph Orndorff; Natalie Williams-Bouyer; Michael Netherland Jr; Nur A Hasan; Md Ibrahim Tahashilder; Jian Sha; Ashok K Chopra; David Reynoso

doi:10.1093/cid/ciae272

Clinical presentation, antimicrobial resistance, and treatment outcomes of Aeromonas human infections: A 14-year retrospective study and comparative genomics of two isolates from fatal cases

Clin Infect Dis. 2024 May 17:ciae272. doi: 10.1093/cid/ciae272. Online ahead of print.

Authors

Roberto Pineda-Reyes^{1

2}, Blake H Neil³, Joseph Orndorff², Natalie Williams-Bouyer⁴, Michael Netherland Jr⁵, Nur A Hasan⁵, Md Ibrahim Tahashilder⁶, Jian Sha^{3

7}, Ashok K Chopra^{3

7}, David Reynoso^{1

2}

Affiliations

¹ Infectious Disease Division, University of Texas Medical Branch, Galveston, TX, USA.
² Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX, USA.
³ Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
⁴ Department of Pathology University of Texas Medical Branch, Galveston, TX, USA.
⁵ EzBiome, Gaithersburg, MD, USA.
⁶ Department of Biostatistics and Data Science, School of Public and Population Health, University of Texas Medical Branch, Galveston, TX, USA.
⁷ Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.

PMID: 38759099
DOI: 10.1093/cid/ciae272

Abstract

Background: Aeromonas virulence may not be entirely dependent on the host immune status. Pathophysiologic determinants of disease progression and severity remain unclear.

Methods: One hundred five patients with Aeromonas infections and 112 isolates were identified, their clinical presentations and outcomes analyzed, and their antimicrobial resistance (AMR) patterns assessed. Two isolates (A and B) from fatal cases of Aeromonas dhakensis bacteremia were characterized using whole genome sequence analysis. Virulence factor- and AMR-encoding genes from these isolates were compared with a well-characterized diarrheal isolate A. dhakensis SSU, and environmental isolate A. hydrophila ATCC_7966T.

Results: Skin and soft tissue infections, traumatic wound infections, sepsis, burns, and intraabdominal infections were common. Diabetes, malignancy, and cirrhosis were frequent comorbidities. Male sex, age ≥ 65 years, hospitalization, burns, and intensive care were associated with complicated disease. High rates of AMR to carbapenems and piperacillin-tazobactam were found. Treatment failure was observed in 25.7% of cases. Septic shock and hospital-acquired infections were predictors of treatment failure. All four isolates harbored assorted broad-spectrum AMR genes including blaOXA, ampC, cphA, and efflux pumps. Only clinical isolates possessed both polar and lateral flagellar genes, genes for various surface adhesion proteins, type 3- and -6 secretion systems and their effectors, and toxin genes, including exotoxin A. Both isolates A and B were resistant to colistin and harbored the mobile colistin resistance-3 (mcr-3) gene.

Conclusions: Empirical therapy tailored to local Aeromonas antibiograms may facilitate more favorable outcomes, while advanced diagnostic methods may aid in identifying correct Aeromonas spp. of significant clinical importance.

Keywords: Aeromonas; antimicrobial resistance; genomics; retrospective study.

© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.