Exposure to cold caused an increase in AChE activity of the different brain regions of both Arvicanthis and Columbia and a decrease in the enzyme activity of Scincus midbrain. Heat exposure provoked variable changes in AChE activity of the various brain regions of the three experimental species. The changes in AChE activities may be one of the mechanisms by which birds and mammals tend to acclimatize themselves to various forms of stress. In reptiles, AChE activity varied with changes in ambient temperature and this is probably due to the adaptive significance of thermally directed changes in enzyme substrate affinity.