Extraction from bone powder: collagen, calcium and phosphate

Scand J Clin Lab Invest. 1984 May;44(3):267-9. doi: 10.3109/00365518409083806.

Abstract

A method for the estimation of the extraction rate is shown and exemplified by preliminary data from extraction of collagen, calcium and phosphate with EDTA and neutral salt solution. The initial extraction rate was high, even with neutral salt solution, and optimal extraction efficiency was obtained within 24 h.

MeSH terms

  • Bone and Bones / analysis*
  • Calcium / isolation & purification*
  • Collagen / isolation & purification*
  • Edetic Acid / pharmacology
  • Humans
  • Methods
  • Phenylmethylsulfonyl Fluoride / pharmacology
  • Phosphates / isolation & purification*
  • Spectrophotometry, Atomic

Substances

  • Phosphates
  • Phenylmethylsulfonyl Fluoride
  • Collagen
  • Edetic Acid
  • Calcium