Chromosome damage and SCE induced by the cytostatic factor homo-aza-steroidal ester of P-bis (2-chloro-ethyl) amino phenyl acetic acid in CHO cells in culture

Environ Mutagen. 1983;5(3):279-83. doi: 10.1002/em.2860050306.

Abstract

The cytogenetic effects of homo-aza-steroidal ester (ASE) on Chinese hamster ovary (CHO) cells in vitro are reported. It was found that treatment of cells with a dose as low as 0.025 micrograms/ml of ASE causes a significant number of abnormal metaphases containing mostly chromatid-type aberrations. Similarly, significant frequencies of SCE were induced with only 0.075 micrograms/ml of ASE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / toxicity*
  • Azasteroids*
  • Cell Line
  • Chromosome Aberrations*
  • Chromosome Disorders*
  • Cricetinae
  • Cricetulus
  • Crossing Over, Genetic / drug effects*
  • Female
  • Mutagenicity Tests
  • Mutagens*
  • Nitrogen Mustard Compounds / toxicity*
  • Ovary
  • Sister Chromatid Exchange / drug effects*

Substances

  • Antineoplastic Agents
  • Azasteroids
  • Mutagens
  • Nitrogen Mustard Compounds
  • 3-(((4-(bis(2-chloroethyl)amino)phenyl)acetyl)oxy)-17a-aza-D-homoandrost-5-en-17-one