Detailed analysis of loss of heterozygosity on chromosome band 17p13 in breast carcinoma on the basis of a high-resolution physical map with 29 markers

M Isomura; A Tanigami; H Saito; Y Harada; T Katagiri; J Inazawa; D H Ledbetter; Y Nakamura

doi:10.1002/gcc.2870090305

Detailed analysis of loss of heterozygosity on chromosome band 17p13 in breast carcinoma on the basis of a high-resolution physical map with 29 markers

Genes Chromosomes Cancer. 1994 Mar;9(3):173-9. doi: 10.1002/gcc.2870090305.

Authors

M Isomura¹, A Tanigami, H Saito, Y Harada, T Katagiri, J Inazawa, D H Ledbetter, Y Nakamura

Affiliation

¹ Department of Biochemistry, Cancer Institute, Tokyo, Japan.

PMID: 7515659
DOI: 10.1002/gcc.2870090305

Abstract

We have constructed a physical map of chromosome band 17p13, using 29 markers that had been localized to 17p13 by means of fluorescence in situ hybridization (FISH) and analysis by pulsed-field gel electrophoresis (PFGE). The map spans nearly 8 Mb of genomic DNA, and the estimated average distance between each marker is roughly 290 kb. The p13 band of chromosome 17 is thought to contain a putative tumor suppressor gene in addition and distal to TP53. Deletion mapping in a large number of breast carcinomas indicated that the tumor suppressor gene lies between the loci defined by cC117-708 (D17S878) and p144D6 (D17S34), which are an estimated 7 Mb apart. Our results should contribute to construction of a contig map of chromosome band 17p13 with cosmid and/or YAC (yeast artificial chromosome) clones, and to isolation of the putative tumor suppressor gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast Neoplasms / genetics*
Chromosome Mapping
Chromosomes, Human, Pair 17* / ultrastructure
DNA, Neoplasm / analysis
Genes, Tumor Suppressor
Genetic Markers
Humans
Hybrid Cells
In Situ Hybridization, Fluorescence
Sequence Deletion*

Substances

DNA, Neoplasm
Genetic Markers