[Study of the transmembrane organization of the C-terminal domain of the alpha subunit of Na+,K+-ATPase using polyclonal antibodies to its perimembrane fragments]

Bioorg Khim. 1994 Aug-Sep;20(8-9):833-41.
[Article in Russian]

Abstract

The fragments 825-842, 868-886, 928-945, 946-967, 962-978 of the alpha-subunit of the porcine Na+,K(+)-ATPase were expressed in E. coli as fusion proteins with human tumour necrosis factor. Polyclonal antibodies were obtained to these fragments. The location of the chosen fragments according to the plasma membrane was determined with the polyclonal antibodies by ELISA on intact, lysed or solubilized pig kidney embryo cells. The fragments 825-842, 928-945, and 962-978 contained intracellular epitopes and did not contain extracellular ones; the fragments 868-886 and 946-967 did not contain any exposed epitopes.

MeSH terms

  • Animals
  • Antibodies / immunology*
  • Cattle
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / chemistry*
  • Epitopes / immunology
  • Escherichia coli / genetics
  • Membrane Proteins / chemistry*
  • Membrane Proteins / immunology
  • Peptide Fragments / chemistry*
  • Peptide Fragments / immunology
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / immunology
  • Sodium-Potassium-Exchanging ATPase / chemistry*
  • Sodium-Potassium-Exchanging ATPase / genetics
  • Sodium-Potassium-Exchanging ATPase / immunology

Substances

  • Antibodies
  • Epitopes
  • Membrane Proteins
  • Peptide Fragments
  • Recombinant Fusion Proteins
  • Sodium-Potassium-Exchanging ATPase