[Isolation and characteristics of an ordered library of transcribed sequences of human chromosome 19 from hybrid human-hamster cells]

D Obradovich; A M Borodin; E P Kopantsev; L L Vagner; S V Volik; O D Ermolaeva; Iu B Lebedev; G S Monastyrskaia; E D Sverdlov

[Isolation and characteristics of an ordered library of transcribed sequences of human chromosome 19 from hybrid human-hamster cells]

Bioorg Khim. 1994 Aug-Sep;20(8-9):919-31.

[Article in Russian]

Authors

D Obradovich, A M Borodin, E P Kopantsev, L L Vagner, S V Volik, O D Ermolaeva, Iu B Lebedev, G S Monastyrskaia, E D Sverdlov

PMID: 7530008

Abstract

A new technique based on Alu-PCR amplification of hn-RNA is described for the extraction of human-specific transcribed sequences from a hybrid cell line. Arrayed library of hn-cDNA was constructed and characterized by sequencing about 80 individual clones. A high enrichment by human-specific sequences (about 95%) was demonstrated.

Publication types

English Abstract

MeSH terms

Animals
Base Sequence
Cell Line
Chromosome Mapping
Chromosomes, Human, Pair 19*
Cricetinae
DNA Primers
Genomic Library*
Humans
Hybrid Cells*
Molecular Sequence Data
RNA / genetics
Repetitive Sequences, Nucleic Acid
Transcription, Genetic*

Substances

DNA Primers
RNA