Absolute levels of mRNA by polymerase chain reaction-aided transcript titration assay

Methods Enzymol. 1993:218:420-45. doi: 10.1016/0076-6879(93)18034-a.

Author

M Becker-André¹

Affiliation

¹ GLAXO Institute for Molecular Biology, Geneva, Switzerland.

PMID: 7685469
DOI: 10.1016/0076-6879(93)18034-a

No abstract available

MeSH terms

DNA
DNA-Directed DNA Polymerase
Electrophoresis, Polyacrylamide Gel / methods
Escherichia coli / genetics
Indicators and Reagents
Mutagenesis
Nucleic Acid Hybridization
Oligodeoxyribonucleotides / isolation & purification
Plasmids
Polymerase Chain Reaction / methods*
RNA, Messenger / analysis*
RNA, Messenger / metabolism
RNA-Directed DNA Polymerase / metabolism
Restriction Mapping
Taq Polymerase
Transcription, Genetic

Substances

Indicators and Reagents
Oligodeoxyribonucleotides
RNA, Messenger
DNA
Taq Polymerase
RNA-Directed DNA Polymerase
DNA-Directed DNA Polymerase