In the present study, the effects of an increased daily dose of a dietary supplement (ATP-E, 0.2 g.kg-1.day-1) on Wingate test performance were examined in 12 men (21 +/- 1.6 years) prior to and following 14 days of supplement and placebo ingestion. A double-blind and counterbalanced design was used. Results revealed higher (p < .007) preexercise blood ATP (95.4 +/- 10.5 mumol.dl-1) for the entire group following 14 days of ATP-E ingestion compared to placebo measures (87.6 +/- 10.9 mumol.dl-1). Mean power (667 +/- 73 W) was higher (p < .008) after 14 days of ATP-E ingestion versus placebo (619 +/- 67 W). Peak plasma lactate was lower (p < .07) after 14 days of ATP-E ingestion (14.9 +/- 2.8 mmol.L-1) compared to placebo (16.3 +/- 1.6 mmol.L-1). These data suggested that the improvement in 30-s Wingate test performance in this group may be related to the increased dose of ATP-E.