Abstract
Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics
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Arabidopsis / metabolism
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Base Sequence
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Caulimovirus
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Cell Nucleus / metabolism
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DNA Primers
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DNA, Bacterial / metabolism
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DNA, Ribosomal / metabolism
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Gene Expression Regulation*
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Genetic Vectors
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Methylation
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Molecular Sequence Data
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Nicotiana / genetics
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Nicotiana / metabolism*
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Plant Leaves
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Plants, Genetically Modified
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Plants, Toxic*
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Plasmids
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Polymerase Chain Reaction
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RNA Processing, Post-Transcriptional*
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Rhizobium
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Sequence Homology, Nucleic Acid
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Transcription, Genetic*
Substances
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DNA Primers
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DNA, Bacterial
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DNA, Ribosomal
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T-DNA