Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation

Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10502-6. doi: 10.1073/pnas.91.22.10502.

Abstract

Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Base Sequence
  • Caulimovirus
  • Cell Nucleus / metabolism
  • DNA Primers
  • DNA, Bacterial / metabolism
  • DNA, Ribosomal / metabolism
  • Gene Expression Regulation*
  • Genetic Vectors
  • Methylation
  • Molecular Sequence Data
  • Nicotiana / genetics
  • Nicotiana / metabolism*
  • Plant Leaves
  • Plants, Genetically Modified
  • Plants, Toxic*
  • Plasmids
  • Polymerase Chain Reaction
  • RNA Processing, Post-Transcriptional*
  • Rhizobium
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic*

Substances

  • DNA Primers
  • DNA, Bacterial
  • DNA, Ribosomal
  • T-DNA