Abstract
The cDNA presumed to encode the rat liver regulatory protein of glucokinase has been expressed in Escherichia coli and a partially soluble protein has been obtained. This recombinant protein was partially purified and found to have the same apparent molecular mass as the regulatory protein purified from rat liver. Like the latter, it inhibited rat liver glucokinase competitively with respect to glucose and its effect was sensitive to fructose 6-phosphate and fructose 1-phosphate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Carrier Proteins*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Fructosephosphates / pharmacology
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Glucokinase / antagonists & inhibitors*
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Glucose / metabolism
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Intracellular Signaling Peptides and Proteins
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Liver / chemistry
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Liver / enzymology
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Molecular Sequence Data
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Molecular Weight
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Protein Biosynthesis*
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Proteins / chemistry
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Proteins / isolation & purification
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Proteins / pharmacology
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Rats
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / pharmacology
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Solubility
Substances
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Carrier Proteins
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Fructosephosphates
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Gckr protein, rat
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Intracellular Signaling Peptides and Proteins
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Proteins
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Recombinant Proteins
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glucokinase regulatory protein
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fructose-6-phosphate
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Glucokinase
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Glucose