Abstract
Monoclonal antibodies TP-1 and TP-3 are of potential utility for the radioimmunodiagnosis of osteosarcoma in both human and canine patients. The V genes of these antibodies were cloned and sequenced and to facilitate radiolabeling of these proteins, the location of the lysine residues within these sequences have been determined. The V-domains of TP-1 contain a total of 12 lysines, 10 in the framework region and 2 in the CDR region, while the V-domains of TP-3 contain a total of 14 lysines, 11 in the framework region and 3 in the CDR regions. Using space-filling models, the availability of each lysine residue for radiolabeling, and potential interference with antigen binding was predicted.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antibodies, Monoclonal / chemistry
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Base Sequence
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Cloning, Molecular / methods
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Crystallography, X-Ray
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DNA Primers
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Dog Diseases
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Dogs
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Genes, Immunoglobulin*
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Humans
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Immunoglobulin Heavy Chains / biosynthesis
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Immunoglobulin Heavy Chains / chemistry
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Immunoglobulin Light Chains / biosynthesis
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Immunoglobulin Light Chains / chemistry
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Immunoglobulin Variable Region / biosynthesis*
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Immunoglobulin Variable Region / chemistry
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Lysine*
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Models, Molecular
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Molecular Sequence Data
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Osteosarcoma / diagnostic imaging*
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Osteosarcoma / immunology*
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Osteosarcoma / veterinary
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Polymerase Chain Reaction
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Protein Conformation*
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Radioimmunodetection*
Substances
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Antibodies, Monoclonal
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DNA Primers
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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Immunoglobulin Variable Region
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Lysine