Abstract
Intracerebroventricularly (i.c.v.)-administered [D-Ala2]deltorphin II (20 micrograms) produced a marked locomotor hyperactivity in male ICR mice. The locomotor hyperactivity induced in response to i.c.v. [D-Ala2]deltorphin II (20 micrograms) was suppressed by pretreatment with naltriben (NTB, 10 micrograms) but not 7-benzylidene naltrexone (BNTX, 1 microgram) and D-Phe-Cys-Tyr-D-Try-Orn-Thr-Phe-Thr-NH2 (CTOP, 100 ng). The influence of antisense oligodeoxynucleotide to delta-opioid receptor mRNA (delta-AS oligo) or a mismatch oligodeoxynucleotide (MM oligo) on the locomotor hyperactivity induced by [D-Ala2]deltorphin II was determined. Groups of mice pretreated i.c.v. with delta-AS oligo (1 microgram), MM oligo (1 microgram) or saline (4 microliters) once a day for 3 days, were injected i.c.v. [D-Ala2]deltorphin II (10 or 20 micrograms) and the locomotor response to [D-Ala2]deltorphin II was measured. The locomotor hyperactivity of i.c.v. [D-Ala2]deltorphin II (10 or 20 micrograms) were significantly suppressed by i.c.v. pretreatment with delta-AS oligo but not MM oligo. The present results indicate that pretreatment with delta-AS oligo suppresses mouse locomotor hyperactivity produced by stimulation of delta 2-opioid receptors in the brain.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Analysis of Variance
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Animals
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Base Sequence
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Benzylidene Compounds / administration & dosage
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Benzylidene Compounds / pharmacology
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Cerebral Ventricles / drug effects
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Cerebral Ventricles / physiology*
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Injections, Intraventricular
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Male
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Mice
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Mice, Inbred ICR
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Molecular Sequence Data
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Motor Activity / drug effects*
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Naltrexone / administration & dosage
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Naltrexone / analogs & derivatives
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Naltrexone / pharmacology
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Narcotic Antagonists / pharmacology
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Oligonucleotides, Antisense / administration & dosage
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Oligonucleotides, Antisense / pharmacology*
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Oligopeptides / administration & dosage
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Oligopeptides / antagonists & inhibitors
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Oligopeptides / pharmacology*
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Receptors, Opioid, delta / antagonists & inhibitors
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Receptors, Opioid, delta / biosynthesis
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Receptors, Opioid, delta / genetics*
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Somatostatin / administration & dosage
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Somatostatin / analogs & derivatives
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Somatostatin / pharmacology
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Thionucleotides
Substances
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Benzylidene Compounds
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Narcotic Antagonists
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Oligonucleotides, Antisense
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Oligopeptides
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Receptors, Opioid, delta
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Thionucleotides
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phenylalanyl-cyclo(cysteinyltyrosyl-tryptophyl-ornithyl-threonyl-penicillamine)threoninamide
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naltrindole benzofuran
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deltorphin II, Ala(2)-
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7-benzylidenenaltrexone
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Somatostatin
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Naltrexone