Abstract
Retinoic acid (RA) has profound suppressive effects on growth and survival of human growth factor-dependent cell line, M07e. Treatment of M07e cells by RA reduced expression of egr-1 gene, while the levels of c-myc gene expression remained similar. Suppression of egr-1 gene expression by RA was dosage-dependent and reached maximum at 4 h after RA addition. The decay of egr-1 mRNA was similar in M07e cells treated with or without RA. The transcriptional activity of the promoter region up to -600 or -480 bp upstream of the egr-1 gene was greatly reduced by RA treatment. These data suggest that biological effects of RA on hematopoietic cells may, in part, be mediated by transcriptional suppression of egr-1 gene through its promoter region within -480 bp.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / genetics
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Early Growth Response Protein 1
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Gene Expression Regulation, Leukemic / drug effects*
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Genes, Immediate-Early / drug effects*
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
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Humans
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Immediate-Early Proteins / biosynthesis*
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Immediate-Early Proteins / genetics
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Leukemia, Megakaryoblastic, Acute / pathology
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Megakaryocytes / drug effects*
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Megakaryocytes / metabolism
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Megakaryocytes / pathology
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Promoter Regions, Genetic / drug effects
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Proto-Oncogene Proteins c-myc / biosynthesis
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Proto-Oncogene Proteins c-myc / genetics
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RNA, Messenger / metabolism
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RNA, Neoplasm / genetics
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Recombinant Proteins / pharmacology
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Stem Cell Factor / pharmacology
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Transcription Factors / biosynthesis*
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Transcription Factors / genetics
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Transcription, Genetic / drug effects
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Tretinoin / pharmacology*
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Tumor Cells, Cultured
Substances
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DNA-Binding Proteins
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EGR1 protein, human
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Early Growth Response Protein 1
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Immediate-Early Proteins
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Neoplasm Proteins
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Proto-Oncogene Proteins c-myc
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RNA, Messenger
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RNA, Neoplasm
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Recombinant Proteins
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Stem Cell Factor
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Transcription Factors
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Tretinoin
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Granulocyte-Macrophage Colony-Stimulating Factor