[Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization]

N G Gurskaia; D A Shagin; K A Luk'ianov; L L Vagner; M S Shtutman; E A Musatkina; E V Moinova; A G Tatosian; S A Luk'ianov; E D Sverdlov

[Cloning cDNA for the ha-SDGF gene from a Syrian hamster cell line with increased metastatic potential using subtractive hybridization]

Bioorg Khim. 1996 Jun;22(6):425-31.

[Article in Russian]

Authors

N G Gurskaia, D A Shagin, K A Luk'ianov, L L Vagner, M S Shtutman, E A Musatkina, E V Moinova, A G Tatosian, S A Luk'ianov, E D Sverdlov

PMID: 8975671

Abstract

Using subtractive hybridization, a cDNA library containing over 50% of clones specific for a highly metastatic cell line was obtained from two hamster embryo fibroblast lines with different metastatic potentials. Most of the clones (83%) contained new sequences. One clone contained the ha-SDGF gene cDNA homologous to SDGF cDNA from rodents. The level of ha-SDGF mRNA expression was considerably higher in the highly metastatic cell line.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amphiregulin
Animals
Cell Line, Transformed
Cloning, Molecular
Cricetinae
DNA, Complementary
EGF Family of Proteins
Glycoproteins*
Growth Substances / genetics*
Humans
Intercellular Signaling Peptides and Proteins*
Mesocricetus
Molecular Sequence Data
Neoplasm Metastasis / genetics
Nucleic Acid Hybridization
Rodentia
Sequence Homology, Amino Acid

Substances

AREG protein, human
Amphiregulin
DNA, Complementary
EGF Family of Proteins
Glycoproteins
Growth Substances
Intercellular Signaling Peptides and Proteins

Associated data

GENBANK/Z66535