Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80

G T Duncan; K Balamurugan; B Budowle; M L Tracey

doi:10.1016/s1050-3862(96)00159-3

Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80

Genet Anal. 1996 Nov;13(5):119-21. doi: 10.1016/s1050-3862(96)00159-3.

Authors

G T Duncan¹, K Balamurugan, B Budowle, M L Tracey

Affiliation

¹ Department of Biology, Florida International University, Miami 33199, USA. gdunca01@servax.fiu.edu

PMID: 9021400
DOI: 10.1016/s1050-3862(96)00159-3

Abstract

The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggest that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a BsoF I restriction site immediately adjacent to the repeat region.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Human, Pair 1
Deoxyribonucleases, Type II Site-Specific
Humans
Microsatellite Repeats*
Polymorphism, Genetic*

Substances

endodeoxyribonuclease Fnu4HI
Deoxyribonucleases, Type II Site-Specific
GANTC-specific type II deoxyribonucleases