A simple method to design PCR primer to detect genomic DNA of parasites and its application to Dirofilaria immitis

Mol Cell Probes. 1996 Dec;10(6):423-5. doi: 10.1006/mcpr.1996.0058.

Abstract

A simple method to design a polymerase chain reaction (PCR) primer for parasites of interest was developed using Dirofilaria immitis as a test sample. The method involved the cloning and sequencing of randomly amplified DNA of the parasite, and designing a primer based on the resulting DNA sequence. Using the primer, DNA fragments of the expected length were amplified by a regular PCR with genomic DNA of Dirofilaria immitis.

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers* / biosynthesis
  • DNA, Helminth / analysis*
  • Dirofilaria immitis / genetics
  • Dirofilaria immitis / isolation & purification*
  • Escherichia coli
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Random Amplified Polymorphic DNA Technique

Substances

  • DNA Primers
  • DNA, Helminth

Associated data

  • GENBANK/U42051