Abstract
A complementary DNA clone has been isolated that encodes a coxsackievirus and adenovirus receptor (CAR). When transfected with CAR complementary DNA, nonpermissive hamster cells became susceptible to coxsackie B virus attachment and infection. Furthermore, consistent with previous studies demonstrating that adenovirus infection depends on attachment of a viral fiber to the target cell, CAR-transfected hamster cells bound adenovirus in a fiber-dependent fashion and showed a 100-fold increase in susceptibility to virus-mediated gene transfer. Identification of CAR as a receptor for these two unrelated and structurally distinct viral pathogens is important for understanding viral pathogenesis and has implications for therapeutic gene delivery with adenovirus vectors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviruses, Human / genetics
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Adenoviruses, Human / metabolism*
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Adenoviruses, Human / physiology
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Amino Acid Sequence
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Animals
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CHO Cells
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Coxsackie and Adenovirus Receptor-Like Membrane Protein
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Cricetinae
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Cytopathogenic Effect, Viral
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Enterovirus B, Human / metabolism*
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Enterovirus B, Human / physiology
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Gene Transfer Techniques
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Genetic Vectors
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HeLa Cells
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Humans
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Molecular Sequence Data
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Receptors, Virus / chemistry
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Receptors, Virus / genetics
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Receptors, Virus / isolation & purification*
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Receptors, Virus / metabolism
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Sequence Alignment
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Transfection
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Virus Replication
Substances
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CLMP protein, human
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Coxsackie and Adenovirus Receptor-Like Membrane Protein
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Receptors, Virus
Associated data
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GENBANK/Y07593
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GENBANK/Y10320