Abstract
Human monoamine (M)-form phenol sulfotransferase (PST) was PCR-cloned and transiently expressed in COS-7 cells. The recombinant enzyme was demonstrated to display not only the previously reported sulfotransferase activity toward dopamine, but also novel manganese-dependent Dopa/tyrosine sulfotransferase activities. These results imply a new functional role of the human M-form PST in the homeostatic regulation of Dopa and tyrosine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arylsulfotransferase / metabolism
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COS Cells
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Deoxyribonuclease EcoRI
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Humans
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Manganese / metabolism*
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Manganese / pharmacology
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Recombinant Proteins / metabolism
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Sequence Homology, Nucleic Acid
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Sulfotransferases / metabolism*
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Tumor Cells, Cultured
Substances
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Recombinant Proteins
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Manganese
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Dopa-tyrosine sulfotransferase
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Sulfotransferases
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Arylsulfotransferase
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Deoxyribonuclease EcoRI