Manganese-dependent Dopa/tyrosine sulfation in HepG2 human hepatoma cells: novel Dopa/tyrosine sulfotransferase activities associated with the human monoamine-form phenol sulfotransferase

Biochim Biophys Acta. 1997 Feb 4;1355(2):102-6. doi: 10.1016/s0167-4889(96)00166-8.

Abstract

Human monoamine (M)-form phenol sulfotransferase (PST) was PCR-cloned and transiently expressed in COS-7 cells. The recombinant enzyme was demonstrated to display not only the previously reported sulfotransferase activity toward dopamine, but also novel manganese-dependent Dopa/tyrosine sulfotransferase activities. These results imply a new functional role of the human M-form PST in the homeostatic regulation of Dopa and tyrosine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arylsulfotransferase / metabolism
  • COS Cells
  • Deoxyribonuclease EcoRI
  • Humans
  • Manganese / metabolism*
  • Manganese / pharmacology
  • Recombinant Proteins / metabolism
  • Sequence Homology, Nucleic Acid
  • Sulfotransferases / metabolism*
  • Tumor Cells, Cultured

Substances

  • Recombinant Proteins
  • Manganese
  • Dopa-tyrosine sulfotransferase
  • Sulfotransferases
  • Arylsulfotransferase
  • Deoxyribonuclease EcoRI