Decreased short-term production of tumor necrosis factor-alpha and interleukin-1beta in human immunodeficiency virus-seropositive subjects

J Infect Dis. 1997 Jun;175(6):1507-10. doi: 10.1086/516488.

Abstract

Tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) were measured by ELISA in 4.5-h, lipopolysaccharide-stimulated whole blood cultures of 347 human immunodeficiency virus type 1-infected patients and 107 healthy seronegative controls. The production of TNF-alpha was decreased in both AIDS and non-AIDS patients, whereas the production of IL-1beta was decreased in AIDS patients only. The production of TNF-alpha and IL-1beta was positively affected by the concentrations of CD14+ monocytes and CD8+ lymphocytes; however, in patients, the concentration of CD4+ lymphocytes and the presence of AIDS had a negative effect on cytokine production as determined by multiple linear regression analysis. It is concluded that low whole blood cytokine production is mainly caused by low numbers of cells, but a functional defect may also exist.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acquired Immunodeficiency Syndrome / blood*
  • Acquired Immunodeficiency Syndrome / immunology
  • CD4 Lymphocyte Count
  • CD8-Positive T-Lymphocytes
  • HIV Seropositivity / blood*
  • HIV Seropositivity / immunology
  • HIV-1*
  • Humans
  • Interleukin-1 / blood*
  • Lipopolysaccharide Receptors / blood
  • Lymphocyte Subsets
  • Monocytes / chemistry
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Viral Load

Substances

  • Interleukin-1
  • Lipopolysaccharide Receptors
  • Tumor Necrosis Factor-alpha