Abstract
A selected RNA (isolate 6) efficiently catalyzes a self-capping reaction with free GDP, yielding the same 5'-capped structure as is formed by protein GTP:RNA guanylyltransferase. This unexplored RNA-catalyzed reaction type involving nucleophilic attack on phosphate by phosphate adds to the variety of possible postsynthetic RNA-catalyzed RNA modifications. The selected RNA requires only Ca2+ for activation and has a broad active pH range of 4.5-9.0. The RNA also has a 5'-pyrophosphatase activity.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Calcium / pharmacology
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Guanosine Diphosphate / metabolism*
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Guanosine Triphosphate / metabolism
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Hydrogen-Ion Concentration
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Hydrolysis
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Molecular Sequence Data
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Nucleotidyltransferases / metabolism
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Pyrophosphatases / metabolism
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RNA / metabolism
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RNA Caps / chemistry
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RNA Caps / metabolism*
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RNA, Circular
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Ribonucleases / metabolism
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Sodium Hydroxide
Substances
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RNA Caps
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RNA, Circular
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Guanosine Diphosphate
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Sodium Hydroxide
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RNA
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Guanosine Triphosphate
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Nucleotidyltransferases
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mRNA guanylyltransferase
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Ribonucleases
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Pyrophosphatases
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Calcium