[Detection of c-Ki-ras oncogene codon 12 point mutations in lung carcinoma, gastric carcinoma and hepatic carcinoma]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1996 Apr;18(2):95-8.
[Article in Chinese]

Abstract

DNA extracted from paraffin-embedded tissues of 30 lung carcinomas, 40 gastric carcinomas and 22 hepatocellular carcinomas was tested for the presence of c-Ki-ras codon 12 point mutation by PCR-RFLP technique. The results showed that 20% (6/30) lung carcinomas and 2.5% (1/40) gastric carcinomas had Ki-ras codon 12 point mutation, but non of the 22 hepatocellular carcinomas displayed this point mutation. Among 6 cases of lung carcinomas of mutation, 4 were squamous carcinomas and 2 were adenocarcinomas.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics*
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Squamous Cell / genetics*
  • Codon / genetics
  • Female
  • Genes, ras / genetics*
  • Humans
  • Liver Neoplasms / genetics*
  • Lung Neoplasms / genetics*
  • Male
  • Middle Aged
  • Point Mutation*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Stomach Neoplasms / genetics*

Substances

  • Codon