Abstract
A yeast 50-kDa mRNA-binding protein (50mRNP) is found selectively associated with the 48S and 80S initiation complexes. This protein is structurally related to the translational elongation factor EF-1alpha. The protein reacts with antibodies directed against EF-1alpha and, similarly, EF-1alpha recognizes antibodies against the 50mRNP protein. This is evidence that they share at least one epitope which allows a similar antigenic behavior. In addition, both proteins show similar cleavage patterns upon treatment with the endoproteinase Lys-C. A murine antibody raised against 50mRNP inhibits both 48S and 80S initiation complex formation. The inhibitory effect is relieved by preincubating anti-50mRNP with EF-1alpha. Antibody to EF-1alpha manifests a similar inhibitory pattern for the formation of 48S and 80S complexes. These data strongly suggest that 50mRNP is an EF-1alpha-like polypeptide essential for the formation of the above complexes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies / immunology
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Centrifugation, Density Gradient
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Electrophoresis, Polyacrylamide Gel
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Epitopes / immunology
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Fungal Proteins / chemistry
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Fungal Proteins / metabolism
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Metalloendopeptidases / metabolism
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Peptide Chain Initiation, Translational
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Peptide Elongation Factor 1
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Peptide Elongation Factors / chemistry
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Peptide Elongation Factors / immunology
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Peptide Elongation Factors / physiology
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Peptide Fragments / isolation & purification
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Peptides / antagonists & inhibitors
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Peptides / metabolism
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Polyribosomes / chemistry
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Polyribosomes / metabolism
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Protein Binding
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RNA, Messenger / genetics
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RNA, Messenger / metabolism*
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RNA-Binding Proteins / chemistry
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RNA-Binding Proteins / metabolism*
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Ribonucleoproteins / chemistry
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Ribonucleoproteins / immunology
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Ribonucleoproteins / metabolism*
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Ribosomes / metabolism*
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae / metabolism*
Substances
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Antibodies
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Epitopes
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Fungal Proteins
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Peptide Elongation Factor 1
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Peptide Elongation Factors
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Peptide Fragments
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Peptides
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RNA, Messenger
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RNA-Binding Proteins
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Ribonucleoproteins
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messenger ribonucleoprotein
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polyphenylalanine
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Metalloendopeptidases
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peptidyl-Lys metalloendopeptidase