Abstract
In this paper we report the cloning and characterization of cDNA encoding a novel, short form of heparin-binding EGF-like growth factor (SF HB-EGF), and show expression of specific mRNA in various tissues and cell types. Our data suggest that SF HB-EGF mRNA is a product of alternative splicing. Like normal HB-EGF, SF HB-EGF contains the signal peptide, the propeptide, the heparin-binding domain and the first two conservative disulfide loops of the EGF unit. Instead of the third disulfide loop, the spacer, the transmembrane and the cytoplasmic domains, SF HB-EGF has a nine amino acid tail.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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DNA Transposable Elements
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DNA, Complementary
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Epidermal Growth Factor / genetics*
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Epidermal Growth Factor / metabolism*
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Gene Expression Regulation
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Heparin-binding EGF-like Growth Factor
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Humans
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Intercellular Signaling Peptides and Proteins
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Microsomes / metabolism
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Molecular Sequence Data
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Protein Precursors / genetics*
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Protein Precursors / metabolism
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RNA, Messenger / biosynthesis
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Sequence Analysis, DNA
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Tissue Distribution
Substances
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DNA Transposable Elements
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DNA, Complementary
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HBEGF protein, human
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Heparin-binding EGF-like Growth Factor
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Intercellular Signaling Peptides and Proteins
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Protein Precursors
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RNA, Messenger
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Epidermal Growth Factor
Associated data
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GENBANK/X89728
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GENBANK/X89729