Expression of mRNA for a short form of heparin-binding EGF-like growth factor

Gene. 1997 Aug 11;195(1):81-6. doi: 10.1016/s0378-1119(97)00161-3.

Abstract

In this paper we report the cloning and characterization of cDNA encoding a novel, short form of heparin-binding EGF-like growth factor (SF HB-EGF), and show expression of specific mRNA in various tissues and cell types. Our data suggest that SF HB-EGF mRNA is a product of alternative splicing. Like normal HB-EGF, SF HB-EGF contains the signal peptide, the propeptide, the heparin-binding domain and the first two conservative disulfide loops of the EGF unit. Instead of the third disulfide loop, the spacer, the transmembrane and the cytoplasmic domains, SF HB-EGF has a nine amino acid tail.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA Transposable Elements
  • DNA, Complementary
  • Epidermal Growth Factor / genetics*
  • Epidermal Growth Factor / metabolism*
  • Gene Expression Regulation
  • Heparin-binding EGF-like Growth Factor
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Microsomes / metabolism
  • Molecular Sequence Data
  • Protein Precursors / genetics*
  • Protein Precursors / metabolism
  • RNA, Messenger / biosynthesis
  • Sequence Analysis, DNA
  • Tissue Distribution

Substances

  • DNA Transposable Elements
  • DNA, Complementary
  • HBEGF protein, human
  • Heparin-binding EGF-like Growth Factor
  • Intercellular Signaling Peptides and Proteins
  • Protein Precursors
  • RNA, Messenger
  • Epidermal Growth Factor

Associated data

  • GENBANK/X89728
  • GENBANK/X89729