Abstract
We have developed a procedure using flow cytometric measurement of a mitosis-specific antigen that may be used to count mitotic cells and sort them from non-mitotic cells. The procedure may also be used in conjunction with measurement of cellular DNA content and of bromodeoxyuridine incorporation into cellular DNA to assign cells to the G1/G0, S, G2, or M phase of the cell cycle.
Copyright 1998 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal
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Antibody Specificity
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Antigens / analysis
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Breast Neoplasms
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Carcinoma
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Cell Separation
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DNA, Neoplasm / analysis
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Flow Cytometry / methods*
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Humans
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Interphase
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Mitosis*
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Nocodazole / pharmacology
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Nucleolin
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Phosphoproteins / analysis
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RNA-Binding Proteins / analysis
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Tumor Cells, Cultured
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tau Proteins / analysis
Substances
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Antibodies, Monoclonal
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Antigens
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DNA, Neoplasm
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Phosphoproteins
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RNA-Binding Proteins
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tau Proteins
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Nocodazole