Abstract
Base-excision of a self-complementary oligonucleotide with central G:T mismatches by the G:T/U-specific mismatch DNA glycosylase (MUG), generates an unusual DNA structure which is remarkably similar in conformation to an interstrand DNA adduct of the anti-tumor drug cis-diamminedichloroplatinum. The abasic sugars generated by excision of the mismatched thymines are extruded from the double-helix, and the 'widowed' deoxyguanosines rotate so that their N7 and O6 groups protrude into the minor groove of the duplex and restack in an interleaved intercalative geometry, generating a kink in the helix axis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Cisplatin / chemistry*
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Computer Simulation
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Crystallography
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DNA Adducts / chemistry*
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DNA Repair*
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Models, Molecular
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Molecular Conformation
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N-Glycosyl Hydrolases / chemistry*
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N-Glycosyl Hydrolases / genetics
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Oligodeoxyribonucleotides / chemistry
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Recombinant Proteins / chemistry
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Thymine DNA Glycosylase*
Substances
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Bacterial Proteins
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DNA Adducts
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Oligodeoxyribonucleotides
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Recombinant Proteins
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N-Glycosyl Hydrolases
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Thymine DNA Glycosylase
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mismatch-specific thymine uracil-DNA glycosylase
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Cisplatin