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CRISPR/Cas9 editing in human pluripotent stem cell-cardiomyocytes highlights arrhythmias, hypocontractility, and energy depletion as potential therapeutic targets for hypertrophic cardiomyopathy.
Eur Heart J. 2018 Nov 14;39(43):3879-3892. doi: 10.1093/eurheartj/ehy249.
Eur Heart J. 2018.
PMID: 29741611
Free PMC article.
Simplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.
Kondrashov A, Duc Hoang M, Smith JGW, Bhagwan JR, Duncan G, Mosqueira D, Munoz MB, Vo NTN, Denning C.
Kondrashov A, et al. Among authors: bhagwan jr.
Stem Cells Dev. 2018 Mar 15;27(6):391-404. doi: 10.1089/scd.2017.0268. Epub 2018 Mar 12.
Stem Cells Dev. 2018.
PMID: 29402189
Free PMC article.
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Isogenic Pairs of hiPSC-CMs with Hypertrophic Cardiomyopathy/LVNC-Associated ACTC1 E99K Mutation Unveil Differential Functional Deficits.
Smith JGW, Owen T, Bhagwan JR, Mosqueira D, Scott E, Mannhardt I, Patel A, Barriales-Villa R, Monserrat L, Hansen A, Eschenhagen T, Harding SE, Marston S, Denning C.
Smith JGW, et al. Among authors: bhagwan jr.
Stem Cell Reports. 2018 Nov 13;11(5):1226-1243. doi: 10.1016/j.stemcr.2018.10.006. Epub 2018 Nov 1.
Stem Cell Reports. 2018.
PMID: 30392975
Free PMC article.
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Modeling Hypertrophic Cardiomyopathy: Mechanistic Insights and Pharmacological Intervention.
Mosqueira D, Smith JGW, Bhagwan JR, Denning C.
Mosqueira D, et al. Among authors: bhagwan jr.
Trends Mol Med. 2019 Sep;25(9):775-790. doi: 10.1016/j.molmed.2019.06.005. Epub 2019 Jul 17.
Trends Mol Med. 2019.
PMID: 31324451
Free article.
Review.
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Isogenic models of hypertrophic cardiomyopathy unveil differential phenotypes and mechanism-driven therapeutics.
Bhagwan JR, Mosqueira D, Chairez-Cantu K, Mannhardt I, Bodbin SE, Bakar M, Smith JGW, Denning C.
Bhagwan JR, et al.
J Mol Cell Cardiol. 2020 Aug;145:43-53. doi: 10.1016/j.yjmcc.2020.06.003. Epub 2020 Jun 10.
J Mol Cell Cardiol. 2020.
PMID: 32531470
Free PMC article.
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Variable expression and silencing of CRISPR-Cas9 targeted transgenes identifies the AAVS1 locus as not an entirely safe harbour.
Bhagwan JR, Collins E, Mosqueira D, Bakar M, Johnson BB, Thompson A, Smith JGW, Denning C.
Bhagwan JR, et al.
F1000Res. 2019 Nov 12;8:1911. doi: 10.12688/f1000research.19894.2. eCollection 2019.
F1000Res. 2019.
PMID: 32789000
Free PMC article.
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