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Stepwise Promoter Melting by Bacterial RNA Polymerase.
Chen J, Chiu C, Gopalkrishnan S, Chen AY, Olinares PDB, Saecker RM, Winkelman JT, Maloney MF, Chait BT, Ross W, Gourse RL, Campbell EA, Darst SA. Chen J, et al. Among authors: saecker rm. Mol Cell. 2020 Apr 16;78(2):275-288.e6. doi: 10.1016/j.molcel.2020.02.017. Epub 2020 Mar 10. Mol Cell. 2020. PMID: 32160514 Free PMC article.
DNA footprints of the two kinetically significant intermediates in formation of an RNA polymerase-promoter open complex: evidence that interactions with start site and downstream DNA induce sequential conformational changes in polymerase and DNA.
Craig ML, Tsodikov OV, McQuade KL, Schlax PE Jr, Capp MW, Saecker RM, Record MT Jr. Craig ML, et al. Among authors: saecker rm. J Mol Biol. 1998 Nov 6;283(4):741-56. doi: 10.1006/jmbi.1998.2129. J Mol Biol. 1998. PMID: 9790837
Solute probes of conformational changes in open complex (RPo) formation by Escherichia coli RNA polymerase at the lambdaPR promoter: evidence for unmasking of the active site in the isomerization step and for large-scale coupled folding in the subsequent conversion to RPo.
Kontur WS, Saecker RM, Davis CA, Capp MW, Record MT Jr. Kontur WS, et al. Among authors: saecker rm. Biochemistry. 2006 Feb 21;45(7):2161-77. doi: 10.1021/bi051835v. Biochemistry. 2006. PMID: 16475805 Free PMC article.
Do sigma factors need help with a meltdown?
Saecker RM, Davis CA, Record MT Jr. Saecker RM, et al. Cell. 2006 Oct 20;127(2):256-8. doi: 10.1016/j.cell.2006.10.009. Cell. 2006. PMID: 17055426 Free article.
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