The anti-Rac1-GTP antibody and the detection of active Rac1: a tool with a fundamental flaw.
Baker MJ, Kazanietz MG.
Baker MJ, et al.
Small GTPases. 2022 Jan;13(1):136-140. doi: 10.1080/21541248.2021.1920824. Epub 2021 Apr 29.
Small GTPases. 2022.
PMID: 33910489
Free PMC article.
Using a series of cancer cellular models, we recently demonstrated that a widely used antibody for visualizing active Rac1 (Rac1-GTP) does not recognize Rac1 but instead recognizes vimentin filaments (Baker MJ, J. Biol. Chem. 295:13698-13710, 2020). We believe that …
Using a series of cancer cellular models, we recently demonstrated that a widely used antibody for visualizing active Rac1 (Rac1-GTP) does n …