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A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike.
Fu Y, da Fonseca Rezende E Mello J, Fleming BD, Renn A, Chen CZ, Hu X, Xu M, Gorshkov K, Hanson Q, Zheng W, Lee EM, Perera L, Petrovich R, Pradhan M, Eastman RT, Itkin Z, Stanley TB, Hsu A, Dandey V, Sharma K, Gillette W, Taylor T, Ramakrishnan N, Perkins S, Esposito D, Oh E, Susumu K, Wolak M, Ferrer M, Hall MD, Borgnia MJ, Simeonov A. Fu Y, et al. Among authors: gillette w. PLoS One. 2022 Aug 10;17(8):e0272364. doi: 10.1371/journal.pone.0272364. eCollection 2022. PLoS One. 2022. PMID: 35947606 Free PMC article.
Gateway cloning is compatible with protein secretion from Pichia pastoris.
Esposito D, Gillette WK, Miller DA, Taylor TE, Frank PH, Hu R, Bekisz J, Hernandez J, Cregg JM, Zoon KC, Hartley JL. Esposito D, et al. Protein Expr Purif. 2005 Apr;40(2):424-8. doi: 10.1016/j.pep.2004.12.006. Protein Expr Purif. 2005. PMID: 15766886 Free PMC article.
Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions.
Gillette WK, Esposito D, Abreu Blanco M, Alexander P, Bindu L, Bittner C, Chertov O, Frank PH, Grose C, Jones JE, Meng Z, Perkins S, Van Q, Ghirlando R, Fivash M, Nissley DV, McCormick F, Holderfield M, Stephen AG. Gillette WK, et al. Sci Rep. 2015 Nov 2;5:15916. doi: 10.1038/srep15916. Sci Rep. 2015. PMID: 26522388 Free PMC article.
102 results