Vernet T - Search Results

1

Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase.

Tessier DC, Brousseau R, Vernet T. Tessier DC, et al. Among authors: vernet t. Anal Biochem. 1986 Oct;158(1):171-8. doi: 10.1016/0003-2697(86)90606-8. Anal Biochem. 1986. PMID: 3799962

2

Contribution of the glutamine 19 side chain to transition-state stabilization in the oxyanion hole of papain.

Ménard R, Carrière J, Laflamme P, Plouffe C, Khouri HE, Vernet T, Tessier DC, Thomas DY, Storer AC. Ménard R, et al. Among authors: vernet t. Biochemistry. 1991 Sep 17;30(37):8924-8. doi: 10.1021/bi00101a002. Biochemistry. 1991. PMID: 1892809

3

A protein engineering study of the role of aspartate 158 in the catalytic mechanism of papain.

Ménard R, Khouri HE, Plouffe C, Dupras R, Ripoll D, Vernet T, Tessier DC, Lalberté F, Thomas DY, Storer AC. Ménard R, et al. Among authors: vernet t. Biochemistry. 1990 Jul 17;29(28):6706-13. doi: 10.1021/bi00480a021. Biochemistry. 1990. PMID: 2397208

4

Codon-based combinatorial alanine scanning site-directed mutagenesis: design, implementation, and polymerase chain reaction screening.

Chatellier J, Mazza A, Brousseau R, Vernet T. Chatellier J, et al. Among authors: vernet t. Anal Biochem. 1995 Aug 10;229(2):282-90. doi: 10.1006/abio.1995.1414. Anal Biochem. 1995. PMID: 7485984

5

Engineering of papain: selective alteration of substrate specificity by site-directed mutagenesis.

Khouri HE, Vernet T, Ménard R, Parlati F, Laflamme P, Tessier DC, Gour-Salin B, Thomas DY, Storer AC. Khouri HE, et al. Among authors: vernet t. Biochemistry. 1991 Sep 17;30(37):8929-36. doi: 10.1021/bi00101a003. Biochemistry. 1991. PMID: 1892810

6

Importance of hydrogen-bonding interactions involving the side chain of Asp158 in the catalytic mechanism of papain.

Ménard R, Khouri HE, Plouffe C, Laflamme P, Dupras R, Vernet T, Tessier DC, Thomas DY, Storer AC. Ménard R, et al. Among authors: vernet t. Biochemistry. 1991 Jun 4;30(22):5531-8. doi: 10.1021/bi00236a028. Biochemistry. 1991. PMID: 2036422

7

Modification of the electrostatic environment is tolerated in the oxyanion hole of the cysteine protease papain.

Ménard R, Plouffe C, Laflamme P, Vernet T, Tessier DC, Thomas DY, Storer AC. Ménard R, et al. Among authors: vernet t. Biochemistry. 1995 Jan 17;34(2):464-71. doi: 10.1021/bi00002a010. Biochemistry. 1995. PMID: 7819238

8

Processing of the papain precursor. Purification of the zymogen and characterization of its mechanism of processing.

Vernet T, Khouri HE, Laflamme P, Tessier DC, Musil R, Gour-Salin BJ, Storer AC, Thomas DY. Vernet T, et al. J Biol Chem. 1991 Nov 15;266(32):21451-7. J Biol Chem. 1991. PMID: 1939177 Free article.

This 39-kDa secreted propapain zymogen molecule is glycosylated and can be processed in vitro into an enzymatically active authentic papain molecule of 24.5 kDa (Vernet, T., Tessier, D.C., Richardson, C., Laliberte, F., Khouri, H. ...

This 39-kDa secreted propapain zymogen molecule is glycosylated and can be processed in vitro into an enzymatically active authentic papain …

9

Modulation of the enzymatic activity of papain by interdomain residues remote from the active site.

Altschuh D, Tessier DC, Vernet T. Altschuh D, et al. Among authors: vernet t. Protein Eng. 1994 Jun;7(6):769-75. doi: 10.1093/protein/7.6.769. Protein Eng. 1994. PMID: 7937707

10

Removal of an inter-domain hydrogen bond through site-directed mutagenesis: role of serine 176 in the mechanism of papain.

Ménard R, Plouffe C, Khouri HE, Dupras R, Tessier DC, Vernet T, Thomas DY, Storer AC. Ménard R, et al. Among authors: vernet t. Protein Eng. 1991 Feb;4(3):307-11. doi: 10.1093/protein/4.3.307. Protein Eng. 1991. PMID: 1907009

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