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Parallel laboratory evolution and rational debugging reveal genomic plasticity to S. cerevisiae synthetic chromosome XIV defects.
Williams TC, Kroukamp H, Xu X, Wightman ELI, Llorente B, Borneman AR, Carpenter AC, Van Wyk N, Meier F, Collier TRV, Espinosa MI, Daniel EL, Walker RSK, Cai Y, Nevalainen HKM, Curach NC, Deveson IW, Mercer TR, Johnson DL, Mitchell LA, Bader JS, Stracquadanio G, Boeke JD, Goold HD, Pretorius IS, Paulsen IT. Williams TC, et al. Among authors: walker rsk. Cell Genom. 2023 Nov 9;3(11):100379. doi: 10.1016/j.xgen.2023.100379. eCollection 2023 Nov 8. Cell Genom. 2023. PMID: 38020977 Free PMC article.
3D organization of synthetic and scrambled chromosomes.
Mercy G, Mozziconacci J, Scolari VF, Yang K, Zhao G, Thierry A, Luo Y, Mitchell LA, Shen M, Shen Y, Walker R, Zhang W, Wu Y, Xie ZX, Luo Z, Cai Y, Dai J, Yang H, Yuan YJ, Boeke JD, Bader JS, Muller H, Koszul R. Mercy G, et al. Science. 2017 Mar 10;355(6329):eaaf4597. doi: 10.1126/science.aaf4597. Science. 2017. PMID: 28280150 Free PMC article.
Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics.
Blount BA, Lu X, Driessen MRM, Jovicevic D, Sanchez MI, Ciurkot K, Zhao Y, Lauer S, McKiernan RM, Gowers GF, Sweeney F, Fanfani V, Lobzaev E, Palacios-Flores K, Walker RSK, Hesketh A, Cai J, Oliver SG, Cai Y, Stracquadanio G, Mitchell LA, Bader JS, Boeke JD, Ellis T. Blount BA, et al. Among authors: walker rsk. Cell Genom. 2023 Nov 9;3(11):100418. doi: 10.1016/j.xgen.2023.100418. eCollection 2023 Nov 8. Cell Genom. 2023. PMID: 38020971 Free PMC article.
Killer yeasts: expanding frontiers in the age of synthetic biology.
Billerbeck S, Walker RSK, Pretorius IS. Billerbeck S, et al. Among authors: walker rsk. Trends Biotechnol. 2024 Apr 3:S0167-7799(24)00067-2. doi: 10.1016/j.tibtech.2024.03.003. Online ahead of print. Trends Biotechnol. 2024. PMID: 38575438 Free article. Review.
Design, construction, and functional characterization of a tRNA neochromosome in yeast.
Schindler D, Walker RSK, Jiang S, Brooks AN, Wang Y, Müller CA, Cockram C, Luo Y, García A, Schraivogel D, Mozziconacci J, Pena N, Assari M, Sánchez Olmos MDC, Zhao Y, Ballerini A, Blount BA, Cai J, Ogunlana L, Liu W, Jönsson K, Abramczyk D, Garcia-Ruiz E, Turowski TW, Swidah R, Ellis T, Pan T, Antequera F, Shen Y, Nieduszynski CA, Koszul R, Dai J, Steinmetz LM, Boeke JD, Cai Y. Schindler D, et al. Among authors: walker rsk. Cell. 2023 Nov 22;186(24):5237-5253.e22. doi: 10.1016/j.cell.2023.10.015. Epub 2023 Nov 8. Cell. 2023. PMID: 37944512 Free article.
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