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Cryo-EM structure of the transposon-associated TnpB enzyme.
Nakagawa R, Hirano H, Omura SN, Nety S, Kannan S, Altae-Tran H, Yao X, Sakaguchi Y, Ohira T, Wu WY, Nakayama H, Shuto Y, Tanaka T, Sano FK, Kusakizako T, Kise Y, Itoh Y, Dohmae N, van der Oost J, Suzuki T, Zhang F, Nureki O. Nakagawa R, et al. Among authors: wu wy. Nature. 2023 Apr;616(7956):390-397. doi: 10.1038/s41586-023-05933-9. Epub 2023 Apr 5. Nature. 2023. PMID: 37020030 Free PMC article.
The miniature CRISPR-Cas12m effector binds DNA to block transcription.
Wu WY, Mohanraju P, Liao C, Adiego-Pérez B, Creutzburg SCA, Makarova KS, Keessen K, Lindeboom TA, Khan TS, Prinsen S, Joosten R, Yan WX, Migur A, Laffeber C, Scott DA, Lebbink JHG, Koonin EV, Beisel CL, van der Oost J. Wu WY, et al. Mol Cell. 2022 Dec 1;82(23):4487-4502.e7. doi: 10.1016/j.molcel.2022.11.003. Epub 2022 Nov 24. Mol Cell. 2022. PMID: 36427491 Free article.
Mechanistic and evolutionary insights into a type V-M CRISPR-Cas effector enzyme.
Omura SN, Nakagawa R, Südfeld C, Villegas Warren R, Wu WY, Hirano H, Laffeber C, Kusakizako T, Kise Y, Lebbink JHG, Itoh Y, van der Oost J, Nureki O. Omura SN, et al. Among authors: wu wy. Nat Struct Mol Biol. 2023 Aug;30(8):1172-1182. doi: 10.1038/s41594-023-01042-3. Epub 2023 Jul 17. Nat Struct Mol Biol. 2023. PMID: 37460897 Free PMC article.
Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F. Zetsche B, et al. Among authors: wu wy. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. Nat Biotechnol. 2017. PMID: 27918548 Free PMC article.
610 results