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The tracrRNA in CRISPR Biology and Technologies.
Liao C, Beisel CL. Liao C, et al. Annu Rev Genet. 2021 Nov 23;55:161-181. doi: 10.1146/annurev-genet-071719-022559. Epub 2021 Aug 20. Annu Rev Genet. 2021. PMID: 34416117 Free PMC article. Review.
Optimized metrics for orthogonal combinatorial CRISPR screens.
Cetin R, Wegner M, Luwisch L, Saud S, Achmedov T, Süsser S, Vera-Guapi A, Müller K, Matthess Y, Quandt E, Schaubeck S, Beisel CL, Kaulich M. Cetin R, et al. Sci Rep. 2023 May 6;13(1):7405. doi: 10.1038/s41598-023-34597-8. Sci Rep. 2023. PMID: 37149686 Free PMC article.
RNA recording in single bacterial cells using reprogrammed tracrRNAs.
Jiao C, Reckstadt C, König F, Homberger C, Yu J, Vogel J, Westermann AJ, Sharma CM, Beisel CL. Jiao C, et al. Nat Biotechnol. 2023 Aug;41(8):1107-1116. doi: 10.1038/s41587-022-01604-8. Epub 2023 Jan 5. Nat Biotechnol. 2023. PMID: 36604543 Free PMC article.
Spacer prioritization in CRISPR-Cas9 immunity is enabled by the leader RNA.
Liao C, Sharma S, Svensson SL, Kibe A, Weinberg Z, Alkhnbashi OS, Bischler T, Backofen R, Caliskan N, Sharma CM, Beisel CL. Liao C, et al. Nat Microbiol. 2022 Apr;7(4):530-541. doi: 10.1038/s41564-022-01074-3. Epub 2022 Mar 21. Nat Microbiol. 2022. PMID: 35314780 Free PMC article.