Transformation and gene editing in the bioenergy grass Miscanthus.
Trieu A, Belaffif MB, Hirannaiah P, Manjunatha S, Wood R, Bathula Y, Billingsley RL, Arpan A, Sacks EJ, Clemente TE, Moose SP, Reichert NA, Swaminathan K.
Trieu A, et al.
Biotechnol Biofuels Bioprod. 2022 Dec 28;15(1):148. doi: 10.1186/s13068-022-02241-8.
Biotechnol Biofuels Bioprod. 2022.
PMID: 36578060
Free PMC article.
Classified as paleo-allopolyploids (duplicated ancient Sorghum-like DNA plus chromosome fusion event), design of guide RNAs (gRNAs) for Miscanthus needed to target both homeologs and their alleles to account for functional redundancy. Prior research in Zea mays demonstrate …
Classified as paleo-allopolyploids (duplicated ancient Sorghum-like DNA plus chromosome fusion event), design of guide RNAs (gRNAs) for Misc …