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Mechanisms of Transcriptional Pausing in Bacteria.
Kang JY, Mishanina TV, Landick R, Darst SA. Kang JY, et al. J Mol Biol. 2019 Sep 20;431(20):4007-4029. doi: 10.1016/j.jmb.2019.07.017. Epub 2019 Jul 13. J Mol Biol. 2019. PMID: 31310765 Free PMC article. Review.
Time-resolved cryo-EM using Spotiton.
Dandey VP, Budell WC, Wei H, Bobe D, Maruthi K, Kopylov M, Eng ET, Kahn PA, Hinshaw JE, Kundu N, Nimigean CM, Fan C, Sukomon N, Darst SA, Saecker RM, Chen J, Malone B, Potter CS, Carragher B. Dandey VP, et al. Nat Methods. 2020 Sep;17(9):897-900. doi: 10.1038/s41592-020-0925-6. Epub 2020 Aug 10. Nat Methods. 2020. PMID: 32778833 Free PMC article.
Polarity of the CRISPR roadblock to transcription.
Hall PM, Inman JT, Fulbright RM, Le TT, Brewer JJ, Lambert G, Darst SA, Wang MD. Hall PM, et al. Nat Struct Mol Biol. 2022 Dec;29(12):1217-1227. doi: 10.1038/s41594-022-00864-x. Epub 2022 Dec 5. Nat Struct Mol Biol. 2022. PMID: 36471058 Free PMC article.
RNA polymerase motions during promoter melting.
Feklistov A, Bae B, Hauver J, Lass-Napiorkowska A, Kalesse M, Glaus F, Altmann KH, Heyduk T, Landick R, Darst SA. Feklistov A, et al. Science. 2017 May 26;356(6340):863-866. doi: 10.1126/science.aam7858. Science. 2017. PMID: 28546214 Free PMC article.
Stepwise Promoter Melting by Bacterial RNA Polymerase.
Chen J, Chiu C, Gopalkrishnan S, Chen AY, Olinares PDB, Saecker RM, Winkelman JT, Maloney MF, Chait BT, Ross W, Gourse RL, Campbell EA, Darst SA. Chen J, et al. Mol Cell. 2020 Apr 16;78(2):275-288.e6. doi: 10.1016/j.molcel.2020.02.017. Epub 2020 Mar 10. Mol Cell. 2020. PMID: 32160514 Free PMC article.
35 results