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Page 1
Creation of a bacterial cell controlled by a chemically synthesized genome.
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC. Gibson DG, et al. Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20. Science. 2010. PMID: 20488990
Creating bacterial strains from genomes that have been cloned and engineered in yeast.
Lartigue C, Vashee S, Algire MA, Chuang RY, Benders GA, Ma L, Noskov VN, Denisova EA, Gibson DG, Assad-Garcia N, Alperovich N, Thomas DW, Merryman C, Hutchison CA 3rd, Smith HO, Venter JC, Glass JI. Lartigue C, et al. Science. 2009 Sep 25;325(5948):1693-6. doi: 10.1126/science.1173759. Epub 2009 Aug 20. Science. 2009. PMID: 19696314
Cloning whole bacterial genomes in yeast.
Benders GA, Noskov VN, Denisova EA, Lartigue C, Gibson DG, Assad-Garcia N, Chuang RY, Carrera W, Moodie M, Algire MA, Phan Q, Alperovich N, Vashee S, Merryman C, Venter JC, Smith HO, Glass JI, Hutchison CA 3rd. Benders GA, et al. Nucleic Acids Res. 2010 May;38(8):2558-69. doi: 10.1093/nar/gkq119. Epub 2010 Mar 7. Nucleic Acids Res. 2010. PMID: 20211840 Free PMC article.
Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling.
Suzuki Y, Assad-Garcia N, Kostylev M, Noskov VN, Wise KS, Karas BJ, Stam J, Montague MG, Hanly TJ, Enriquez NJ, Ramon A, Goldgof GM, Richter RA, Vashee S, Chuang RY, Winzeler EA, Hutchison CA 3rd, Gibson DG, Smith HO, Glass JI, Venter JC. Suzuki Y, et al. Genome Res. 2015 Mar;25(3):435-44. doi: 10.1101/gr.182477.114. Epub 2015 Feb 5. Genome Res. 2015. PMID: 25654978 Free PMC article.
Design and synthesis of a minimal bacterial genome.
Hutchison CA 3rd, Chuang RY, Noskov VN, Assad-Garcia N, Deerinck TJ, Ellisman MH, Gill J, Kannan K, Karas BJ, Ma L, Pelletier JF, Qi ZQ, Richter RA, Strychalski EA, Sun L, Suzuki Y, Tsvetanova B, Wise KS, Smith HO, Glass JI, Merryman C, Gibson DG, Venter JC. Hutchison CA 3rd, et al. Science. 2016 Mar 25;351(6280):aad6253. doi: 10.1126/science.aad6253. Science. 2016. PMID: 27013737
One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9.
Kannan K, Tsvetanova B, Chuang RY, Noskov VN, Assad-Garcia N, Ma L, Hutchison Iii CA, Smith HO, Glass JI, Merryman C, Venter JC, Gibson DG. Kannan K, et al. Among authors: assad garcia n. Sci Rep. 2016 Aug 4;6:30714. doi: 10.1038/srep30714. Sci Rep. 2016. PMID: 27489041 Free PMC article.
Essential genes of a minimal bacterium.
Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC. Glass JI, et al. Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):425-30. doi: 10.1073/pnas.0510013103. Epub 2006 Jan 3. Proc Natl Acad Sci U S A. 2006. PMID: 16407165 Free PMC article.
Targeted chromosomal knockouts in Mycoplasma pneumoniae.
Krishnakumar R, Assad-Garcia N, Benders GA, Phan Q, Montague MG, Glass JI. Krishnakumar R, et al. Appl Environ Microbiol. 2010 Aug;76(15):5297-9. doi: 10.1128/AEM.00024-10. Epub 2010 Jun 11. Appl Environ Microbiol. 2010. PMID: 20543037 Free PMC article.
Polar Effects of Transposon Insertion into a Minimal Bacterial Genome.
Hutchison CA 3rd, Merryman C, Sun L, Assad-Garcia N, Richter RA, Smith HO, Glass JI. Hutchison CA 3rd, et al. Among authors: assad garcia n. J Bacteriol. 2019 Sep 6;201(19):e00185-19. doi: 10.1128/JB.00185-19. Print 2019 Oct 1. J Bacteriol. 2019. PMID: 31262838 Free PMC article.
23 results