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Enzymatic activity of the Staphylococcus aureus SplB serine protease is induced by substrates containing the sequence Trp-Glu-Leu-Gln.
J Mol Biol. 2008 May 30;379(2):343-56. doi: 10.1016/j.jmb.2008.03.059. Epub 2008 Apr 3.
J Mol Biol. 2008.
PMID: 18448121
Structural and functional characterization of SplA, an exclusively specific protease of Staphylococcus aureus.
Stec-Niemczyk J, Pustelny K, Kisielewska M, Bista M, Boulware KT, Stennicke HR, Thogersen IB, Daugherty PS, Enghild JJ, Baczynski K, Popowicz GM, Dubin A, Potempa J, Dubin G.
Stec-Niemczyk J, et al. Among authors: boulware kt.
Biochem J. 2009 May 1;419(3):555-64. doi: 10.1042/BJ20081351.
Biochem J. 2009.
PMID: 19175361
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Protease specificity determination by using cellular libraries of peptide substrates (CLiPS).
Boulware KT, Daugherty PS.
Boulware KT, et al.
Proc Natl Acad Sci U S A. 2006 May 16;103(20):7583-8. doi: 10.1073/pnas.0511108103. Epub 2006 May 3.
Proc Natl Acad Sci U S A. 2006.
PMID: 16672368
Free PMC article.
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Evolutionary optimization of peptide substrates for proteases that exhibit rapid hydrolysis kinetics.
Boulware KT, Jabaiah A, Daugherty PS.
Boulware KT, et al.
Biotechnol Bioeng. 2010 Jun 15;106(3):339-46. doi: 10.1002/bit.22693.
Biotechnol Bioeng. 2010.
PMID: 20148412
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Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands.
Rice JJ, Schohn A, Bessette PH, Boulware KT, Daugherty PS.
Rice JJ, et al. Among authors: boulware kt.
Protein Sci. 2006 Apr;15(4):825-36. doi: 10.1110/ps.051897806.
Protein Sci. 2006.
PMID: 16600968
Free PMC article.
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SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability.
Heinzelman P, Snow CD, Smith MA, Yu X, Kannan A, Boulware K, Villalobos A, Govindarajan S, Minshull J, Arnold FH.
Heinzelman P, et al.
J Biol Chem. 2009 Sep 25;284(39):26229-33. doi: 10.1074/jbc.C109.034058. Epub 2009 Jul 22.
J Biol Chem. 2009.
PMID: 19625252
Free PMC article.
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