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The kinetics, substrate, and inhibitor specificity of the monocarboxylate (lactate) transporter of rat liver cells determined using the fluorescent intracellular pH indicator, 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein.
Jackson VN, Halestrap AP. Jackson VN, et al. Among authors: halestrap ap. J Biol Chem. 1996 Jan 12;271(2):861-8. doi: 10.1074/jbc.271.2.861. J Biol Chem. 1996. PMID: 8557697 Free article.
Differences in stereoselectivity were also detected; both carriers showed a lower Km for L-lactate than D-lactate, while hepatocyte MCT exhibited a lower Km for D- than L-2-chloropropionate and for L- than D-3-hydroxybutyrate; this is not the case for MCT1. ...The p …
Differences in stereoselectivity were also detected; both carriers showed a lower Km for L-lactate than D-lactate, while hepatocyte M …
N-terminal protein sequence analysis of the rabbit erythrocyte lactate transporter suggests identity with the cloned monocarboxylate transport protein MCT1.
Poole RC, Halestrap AP. Poole RC, et al. Among authors: halestrap ap. Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):755-9. doi: 10.1042/bj3030755. Biochem J. 1994. PMID: 7980443 Free PMC article.
The process of purification of the 40-50 kDa transporter, labelled with 4,4'-diisothiocyanostilbene-2,2'-disulphonate (DIDS), was followed by Western blotting with anti-DIDS antibodies [Poole, R. C. and Halestrap, A. P. (1992) Biochem. J. 283, 855-862]. Fractions hi …
The process of purification of the 40-50 kDa transporter, labelled with 4,4'-diisothiocyanostilbene-2,2'-disulphonate (DIDS), was followed b …
212 results